Materials and Methods

• 2 x 3 x 5 factorial

• 2 levels of cycling temps: -3/+3C or –8/+8C

• 3 levels of cycling duration:

24h (-3, +3, -3) or (-8, +8, -8)

72h (-3, +3, -3) or (-8, +8, -8)

24h -3C only or -8C only

• 5 treatment temps: +4, -5, -8, -11, -14C

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